Protocols

Quantitative PCR 

Contains:

Measuring cell concentration using Albumin

Measuring total HIV DNA using RU5 

Measuring HIV integrated DNA using Alu-Gag

PBMC Isolation by Ficoll

PBMC isolation from leukapak, whole blood, or red cell exchange waste product using Ficoll Density Gradient Separation. 

O'Doherty Lab GitHub

Visit our lab GitHub Page which will be updated with current codes used by the laboratory for analysis of proviral, TCR, and integration site sequences. 

Code for Clonal Dynamics in HIV Manuscript

Visit this GitHub Repository to access the code utilized in our manuscript Clonal Dynamics of HIV-infected and uninfected T cells  published in eBioMedicine in March 2026!